In vitro Evaluation of Anti-Leishmanial Activities of Zataria Multiflora Boiss, Peganum Harmala and Myrtus Communis by Colorimetric Assay

Barati M., Sharifi I., Sharififar F., (2015) In vitro Evaluation of Anti-Leishmanial Activities of Zataria Multiflora Boiss, Peganum Harmala and Myrtus Communis by Colorimetric Assay. Journal of Kerman University of Medical Sciences. ISSN 1023-9510

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Abstract

Abstract: Background & Aims: Plant extracts and plant-derived compounds are valuable sources of new medicinal agents that are commonly used to treat infectious diseases. The purpose of this study was to evaluate, anti-Leishmanial activity of three plant extracts on L.major promastigotes by colorimetric assay as compared to a trivalent antimonial compound (tartar emetic). Methods: Leishmania major promastigotes were cultured at 25±2°C in stationary phase in RPMI-1640 medium supplemented with 10% heat inactivated fetal calf serum (FCS) and antibiotic. Then by using MTT assay, the biological activity of plant extracts in comparison to potassium antimonyl tartrate [Sb (III)] on L.major promastigotes were assessed. The optical density (OD) due to cleavage of the tetrazolium salt MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] into a colored product formazan by the parasite was measured using ELISA reader and IC<sub>50</sub> values (50% inhibitory concentrations) were determined. All experiments were repeated in duplicate. Results: plant extracts and Tartar emetic inhibited the growth of promastigote forms of L.major in vitro after 72 hours of incubation. IC<sub>50</sub> of tartar emetic was 4.7 µg/ml, and IC<sub>50</sub> values for Zataria multiflora Boiss, Peganum harmala and Myrtus communis extracts were 7.4, 7.2 and 5.8 µg/ml, respectively. Although tartar emetic was more effective than plant extracts, all extracts had profound effects on promastigotes of L.major. Conclusion: Since, plant extracts of Zataria multiflora Boiss, Peganum harmala and Myrtus communis have anti-Leishmanial effects in vitro, further works are required to evaluate the exact effect of these extracts on Leishmania agents in animal models. Keywords: Leishmania major, Zataria multiflora Boiss, Peganum, Myrtle communis , Plant extracts, MTT formazan

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Depositing User: journals journals journals
Date Deposited: 26 Dec 2015 05:34
Last Modified: 26 Dec 2015 05:34
URI: http://eprints.kmu.ac.ir/id/eprint/23094

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